Synthesis of Isotope labeled Trichlorfon-methoxyl-D6 and Dichlorophos-methoxyl-D6
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Abstract
The efficient synthesis of two labeled standards trichlorfon-methoxyl-D6 and dichlorophos- methoxyl-D6 with CD3OD as the precursor were presented. Esterification of phosphorus trichloride with CD3OD provided dimethyl phosphate-methoxyl-D6, which was used to condense with trichloroaldehyde to give trichlorfon-methoxyl-D6. Ttrichlorfon-methoxyl-D6 was further converted to dichlorophos-methoxyl-D6 by alkaline hydrolysis and rearrangement. The yields of trichlorfon-methoxyl-D6 and Dichlorophos-methoxyl-D6 were 57.7 % and 47.3% respectively based on CD3OD consumed. Both products were characterized by HPLC, MS, and NMR to be target compounds. Their chemical purities and isotopic enrichment were higher than 98.0%. The above compounds could be used as internal standards in the food safety field.
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