Radioiodine-labeling of EGCG and Its Biodistribution in Mice
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Abstract
To establish the 125I-EGCGlabeling method and investigate the biodistribution of 125I-EGCG inmice, 125I-EGCG was prepared by Iodogen solid labeling method, andwere isolated and purified by Sephadex-G25 agarose, The labeling yield andradiochemical purity of 125I-EGCG was analyzed by polymide TLC. Thelabeling yield of 125I-EGCG was 89.4% and its radiochemicalpurity(RCP) were 96.4%. The Biodistribution of 125I-EGCG in mice wasmeasured at different times after caudal vein injection with 185 kBq for eachmice. The biodistribution in mice demonstrated that 125I-EGCG wasdistributed into broad organs and tissuuues, especially in the Stomach, Smallintestine and Submaxillay gland, and the biggest uptake of 125I-EGCGin there organs was 15.92、5.83and 11.56 %ID·g-1respectively at 15 min post injection. In addition, 125I-EGCG wascleared out from blood guickly, and the uptake of 131I-EGCG in blood was 11.95 at 5 min, anddecreased to 1.25 at 4 h post injection. Therefore, 125I-EGCG wasstable and it was metabolized mainly in Stomach, Small intestine, Submaxillaygland, worthy of further investigation to trace the compound in vivo and invitro.
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