Lymph Nodes Distribution and Imaging Study of ⁹⁹Tc^m Labeled Dextran Conjugate DCM-1

To evaluate the potential application of ⁹⁹Tc^m labeled mannosylated dextran conjugates with S-Cysteine（Dextran-S-Cysteine-Mannose，DCM）for sentinel lymph node（SLN）imaging，⁹⁹Tc^m-(CO)₃-DCM-1 was prepared via ⁹⁹Tc^m(CO)₃⁺ precursor synthesized by Isolink kit. Then, the effect of injected dosage on SLN uptake was studied. The result of biodistribution demonstrated that the biological behaviour of ⁹⁹Tc^m-(CO)₃-DCM-1 was very sensitive to the injected dosage. When the injected dosage decreased, the uptake of SLN and the PE% increased correspondingly. The result of SLN SPECT imaging study was in accordance with that of biodistribution study. High SLN uptake and PE% of ⁹⁹Tc^m-(CO)₃-DCM-1 showed its promising properties as SLN imaging agent and it was worth to have further investigation.