Determination of Medronic Acid by Reversed-Phase High-Performance Liquid Chromatography

An isocratic liquid chromatographic method with evaporative light scattering detection(ELSD) was developed for the determination of medronic acid and its related substances. Volatile pentylamine was used as the ion-pairing agent. Separations were performend on a Symmetry C8 column with an mobile phase of 98∶2(V∶V) 30 mmol/L pentylamine(pH 5.0,adjusted with acetic acid) methanol. The mobile phase was delivered at a flow-rate 1.0 mL/min. The method is applicable to the routine analysis and the quality control of medronic acid.