Synthesis of ¹³C₆-1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose

With concentrated sulfuric acid as the catalytic agent, ¹³C₆-1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose was synthesized with D-glucose-¹³C₆ and acetone. ¹³C labeled 1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose was characterized by GC and MS. The reaction conditions were optimized to be as follows: the molar ratio of D-glucose-¹³C₆∶acetone = 1∶73.5, the volume of concentrated sulfuric acid = 10 mL, the reaction temperature = 25 ℃, and the reaction time = 12 h. Under these conditions, the yield of ¹³C₆-1,2:5,6-Di-O-isopropylidene-α-D-glucofuranose was 75.6% (based on D-glucose-¹³C₆), with 99.2% ¹³C atom abundance and 98.7% chemical purity.