Labeling of Octreotide With ¹³¹I and Its Vitro Uptake in Inflammatory Cells for Graves’ Ophthalmopathy

Phenylalanine was displaced by tyrosine in octreotide(Tyr₃octreotide) to study the labeling methods of Tyr₃octreotide with ¹³¹I，and the difference uptake ability of ¹³¹I-Tyr₃octreotide in various cell lines of Graves ophthalmopathy were observed. The results showed that Tyr₃octreotide could be labeled with ¹³¹I by chloramines-T method and the optimal conditiona was 1.6 μg chloramines-T and 1.4 μg Tyr₃octreotide. The labeling efficiency was 85%. T lymphocyte, fibroblast, and HUVEC could accumulate ¹³¹I-Tyr₃octreotide largely compared to lipocyte, which indicated that ¹³¹I-Tyr₃octreotide could be combined with inflammatory cells in Graves’ ophthalmopathy.