Synthesis and Characterization of ¹³C₃-Tristearin

A highly efficient synthesis of ¹³C₃ labeled triglycerides of stearic acids from ¹³C₃-glycerol and stearic acids, by immobilized lipase-catalyzed in solvent-free medium was described. The structure of the product were characterized by fourier transform infrared spectrum (FT-IR), nuclear magnetic resonance (NMR), mass spectra (MS). The results showed that triglycerides of stearic acids contained three ¹³C atoms. The isotope abundance of ¹³C₃-tristearin was more than 99% and the yield was 80% of ¹³C₃-tristearin through calculation. Chemical purity (>98%) was obtained by differential scanning calorimetry (DSC).