Characterization of Methacetin-methoxy-¹³C

Methacetin-methoxy-¹³C was synthesized by using methanol-¹³C with a novel method, and the characterization of it was performed using HPLC, LC-MS and ¹HMNR. The results indicated that the synthetic was right. And the yield of methacetin-methoxy-¹³C was 70.0% with 99% ¹³C abundance and 99.8% purity. Compared with the classical method， there was more benefit. The methacetin ¹³C-breath test was performed with the synthetic on the live mice, which showed a precise reflection of alteration of liver function in liver injury and functional recovery.