Labeling of L-leucine With 15N
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Abstract
15N-L-leucine was prepared by fermentation on the basis of high-yielding strain TLU53-8 breeded by breeding prescription,fermentation condition and purification of 15N-L-leucine were studied. The results showed that 15N-L-leucine can be produced with more than 18 g/L. The 15N enrichment of 15N-L-leucine was more than 98%, and the product purity was more than 99%. The results demonstrated that the technique was suitable for producing high quality 15N-L-leucine.
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