The Investigation of Direct Labeling Antibody with 188Re

For more easily and quickly labeling each antibody with 188Re, the method of direct labeling IgG with 188Re was studied here. The direct labeling is conducted at pH5.0 with 2-ME and SnCl2 as the reductant of antibodies and 188ReO4- respectively, and Sodium gluconate as chelate agent. The labeling process is easily and conveniently handled. The labeling efficiency is high (95%) and further purification does not be needed. The labeled antibodies are stable in vitro. Therefore the direct labeling approach has more practical application prospects than other labeling methods.