Production of Monoclonal Antibodies against Enrofloxacin and the Establishment of Enzyme-linked Immumosobent Assay

Mice were immunized with ENRO-BSA conjugate, and seven stable cell lines excreting monoclonal antibodies against ENRO were obtained by hybridoma technique. Then ENRO19B10 was selected to develop an ELISA to analyze enrofloxacin residues in animal-based food products. The standard line of the assay was in the rang 0.5-50 ng/mL. The sensitivity was 0.2 μg/mL. The intra-and intro-assay CVs of 3 samples were lower than 10% and 20% respectively. The average recoveries of chicken muscle tissues, fish, shrimp and honey were in the range of 95.5 %~107.5 %，80.0 %~101.3 %，105.7 %~122.7 % and 93.3 %~111.3 % respectively.