Tissue distribution and excretion of radioactivity in mice after intragastric administration of 3H-beta, beta-dimethylacrylshikonin

Abstract: Tissue distribution and excretion of radioactivity in mice after intragastric administration of 3H-beta, beta-dimethylacrylshikonin were investigated. Tissues and feces were combusted by sample Oxidizer, the subsequent radioactivity measurements were carried out after addition of scintillator to the combusted samples and urine. The results showed that the radioactivity in gastrointestinal system was higher than that in the liver, lung, kidney and heart, while that in skeletal muscle, spinal cord, brain was low. After single intragastric administration (chemical dose 84mg/kg，radioactive dose 22.3MBq/kg) of beta, beta-dimethylacrylshikonin to mice, the cumulative radioactivity rate of urine and feces were（6.810.18）% and（72.879.92）% respectively within 336h, the total excretion rate was（79.68±0.18）%. The plasma, feces and urine were analyzed by HPLC, the results showed that parent drug mainly existed in feces, no parent drug was detected in plasma and urine. The results showed that 3H-beta, beta-dimethylacrylshikonin was distributed widely in mice, and was excreted mainly by fecal route and secondarily via urine.