Synthesis and MicroPET image of 18F-FLT

Abstract Synthesis，stability study and MicroPET image of proliferation imaging agent 3’-deoxy-3’-18F fluorothymidine（18F-FLT）were reported. Nucleophilic substitution of fluoro replacement reaction was proceeded with N-BOC-FLT as labeling precursor. Radiochemical purity(RCP)was determined by high pressure liquid chromatography(HPLC)．Biodistribufion was performed in mice． RCP determined by HPLC was over 95％ and were stable within 6 h. Biodistribufion studies in mice showed that the uptake of 18F-FLT in kidney, spleen and intestine was higher than that of 18F-FLT in heart, liver, lung and bladder at 60min postinjection. MicroPET image of tumor in nude mice bearing tumor xenografts was clear.