Bio-distribution of 125I labeled macrophage migration inhibitory factors in inflammatory model of mice

Abstract: Objective: To evaluate the usefulness of 125I labeled macrophage migration inhibitory factors (MIF) for the scintigraphic imaging of inflammation. Methods: 125I-MIF was radio-iodinated by Iodogen solid labeling method. The stability of 125I- MIF and its bio-distribution in inflammatory model of mice were studied. Results: The labeling rate of 125I- MIF was 96.5%. It was stable within 48 hours at room temperature. The bio-distribution results showed that the 125I- MIF was metabolized by the liver, the radioactivity clearance mainly happened in the kidney and the speed of the blood clearance is rapid. After caudal vein injection with 125I-MIF, the ratio of %ID between inflammatory limb (target) and contralateral healthy limb (non target)( T /NT) were 1.42, 1.35, 2.18 and 2.05 at 0.5, 1, 6, 24 hour respectively. Conclusion: 125I-MIF has the capability of locating the inflammatory foci. The advance of it is more obviously at the late stage than at the early stage. The 125I-MIF may be a potential agent for the diagnosis of concealed and subacute inflammatory disease.