Bioactivity assays and application of 125I radiolabeled human mouse chimeric anti-CD22 monoclonal antibody, SM03

Abstract: AIM To investigate the bioactivity and application of 125I radiolabed human mouse chimeric monoclonal SM03. METHODS: SM03 was radiolabeled with Indogen method. The radiolabled mixture was purified by Sephacryl S-300 HR separation chromospectry. We determined the purity and concentration of separated fraction. The radiolabeled SM03 was applied to screen which cell line express maximum CD22 antigen. RESULTS: The purity and recovery of radiolabeled SM03 were >99% and >47%, respectively. The bioactivity of 125I labeled SM03 and non-labeled SM03 did not have significant difference in statistics. Ramos cell line had the strongest special radioactivity when bound with SM03 in Raji, Daudi and Ramos cell lines. CONCLUSION: Human mouse chimeric anti-CD22 monoclonal antibody can be radiolabeled with Indogen method. The radiolabel will not affect the activity of SM03.