Effect of Fed-batch on Synthesis of 15N L-tryptophan from Precursor Fermentation
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Abstract
Using Candida utilis AS60 as 15N L-tryptophan producing strain,the influence by different feeding modes of glucose, 15N (NH4)2SO4 and 15N anthranilic acid was studies in the paper. The results of these experiments show the yield of 15N L-tryptophan was 3.073g/L by addition of 50 g/L of glucose, 2.1 g/L of 15N (NH4)2SO4 and 1.5 g/L of 15N anthranilic acid after 36 h of fermentation.
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