Establishment of an Enzyme Linked Immunosorbent Assay for Insulin

Two monoclonal antibodies against insulin are applied in two-site enzyme linked immunosorbent assay(ELISA).One of the antibodies is immobilized on the microtiter plate, the other is labelled with HRP.The sensitivity of the assay is 1.7mIU/L and the recovery is 96.4％～111.1 ％.The intraassay and interassay CV are below 11％ and 15.0％
respectively.The correlation coefficient between measured and expected
values is 0.998 after serial dilutions of the samples using high concentration of insulin.The value for normal samples (n=38) is 3.6～
10.8mIU/L.Compared with RIA,the correlation coefficient is acceptable (r=0.97)