The Establishment of an Competition Inhibition (ci) ELISA for sulfamethoxazole

A sulfamethoxazole-specific ELISA was developed to analyze sulfamethoxazole The preparation of horseradish peroxidase labeled sulfamethoxazole（SMX）and polyclonal antibody coated plates are described.The principle for the measurement of SMX is that the SMX in the sample and the enzyme-labeled SMX competitively is bound to the polyclonal antibody against the SMX in the solid phase, And the signal falls with the increasing SMX concentration. The sensitivity of the assay is 0.067μg/L.The intra-and intro-assay CVs are less than 10% and 20% respectively. The analytical recoveries rate of milk, meat, egg and honey are in the range of 94.9%~116.0%,82.0%~107.6%,73.0%~90.0%and61.9%~94.0%respectively,A sulfamethoxazole-specific ELISA is established to analyze SMX residues in animal-based food products.