Establishment of an Enzyme Linked Immunoassay for Chloramphenicol

A sensitive and specific EIA for chloramphenicol(CAP) is established by using one anti-CAP polyclonal antibody.The antibody is coated on the microtiter plate.The labelled compound is horseradish peroxidase(HRP) conjugate of chloramphenicol.TMB-H_2O_2 solution is used as the substrate of HRP.The sensitivity of the assay is 0.046 μg/L.The intra-and inter-CVs of 10 samples are lower than 10% and 24%,respectively.The analytical recovery is 62%～145%.The correlative equation is y=3.988x-0.234,r=0.999,using milk supernatant as buffer.The analytical range is 0.1～10 μg/L.