Experimental Study of ~(99)Tc~m-DTPA-DG Uptake in Lung Cancer A549 Cell

The cellular uptake of ~(99)Tc~m-diethylenetriaminepentaacetic acid-deoxyglucose((~(99)Tc~m)-DTPA-DG) by lung cancer A549 cell are studied.Cellular uptake ratio of ~(99)Tc~m-DTPA-DG is observed in lung cancer A549 cell.The control group is ~(99)Tc~m-DTPA.The results of uptake kinetics show that ~(99)Tc~m-DTPA-DG has maximal uptake at 2 h after incubation in lung cancer A549 cell.The uptake of ~(99)Tc~m-DTPA-DG is significantly higher than that of ~(99)Tc~m-DTPA between 30 min and 2 h after incubation in lung cancer A549 cell(P<0.01).Blood glucose level of mice increases with DTPA-DG injection and decreases with insulin administration.It indicates that ~(99)Tc~m-DTPA-DG could be a imaging agent of glucose metabolism of tumor.