Basic Hydrolysis of 1,3,4,6-tetra-O-acetyl-2-~(18)F fluoro-D-glucose on Solid Phase Extraction

A new base hydrolysis method are used for 1,3,4, 6-tetra-O-acetyl-2-18Ffluoro-D-glu-cose on solid phase extraction. The labeled intermediate is trapped on an active C-18 solid phase extraction cartridge, and hydrolyzed in cartridge with 1 mL 2 mol/L NaOH at room temperature. The results show that there are over 99% of the labeled intermediate being turned into 18F-FDG within 2 rain. It is easy to get 18F-FDG after neutralized with phosphate buffer, purified by C-18 and Alumina cartridge. The basic hydrolysis on solid extraction is a simple method for preparation of 18F-FDG.