The Establishment of an Enzyme Linked Immunosorbent Assay for Neonatal Thyrotropin

A sensitive and specific ELISA for neonatal thyrotropin(Neonatal TSH) is established by using two antiTSH monoclonal antibodies. One of them is coated on the microtiter plate, and the other is biotinlated. The label is horseradish peroxidase(HRP) conjugate of streptavidin. TMBH2O2 solution is used as the substrate of HRP. The sensitivity of the assay is 0.5 mIU/L. The intraand introassay CVs of 3 samples are lower than 15% and 20% respectively. The analytical recovery is in the range from 90.1% to 103.3%. Compared with IRMA, the correlative equation is y=1.01x+0.34, and r=0.873. This method for measuring neonatal TSH is rapid, sensitive, convenient and inexpensive. It is suitable for clinical and research application.