Comparation of the Expression of Insulin Receptor to H22 Hepatoma Cells With Normal Liver Cells of Mice

Insulin is labeled with 125 I by Ch T method. Receptor binding assay of 125 I insulin to H22 hepatoma cells and normal mice liver cells are performed respectively. Binding data are calculated according to Scatchard analysis using the ligand program.Statistical comparison is made with the paired t test. K d values of H22 hepatoma cells and normal mice liver cells are 1.81± 0.56 nmol/L and 2.10±0.91 nmol/L respectively, values of B max are 5.61±1.10 amol per cell and 3.22±0.81 amol per cell respectively. H22 hepatoma cells have a significantly higher B max than normal mice liver cells( P <0.05), while the two K d values have little difference( P >0.05).