THE ASSAY OF GLUCOCORTICOID RECEPTOR OF PERIPHERAL LEUKOCYTES USING RADIOLIGAND BINDING ASSAY——DIAGNOSTIC VALUE IN LEUKEMIA TREATMENT AND PROGNOSIS

The method of measuring the glucocorticoid receptor (GCR) is studied by using whole peripheral leukocytes, and the GCR level of 29 normal cases and 80 cases of acute leukemia are measured. The Kd are 5. 1±2. 01 nmol/1 and 5. 38±1. 21 nmol/1 respectively. The study is emphasized in the relationship between GCR level and the effect of chemotherapy or its short-term prognosis, the result indicates that patients with GCR higher than 6000 site/cell have good short-term prognosis (16/16), whereas less than 6000 site/cell have poor short-term prognosis (14/14), and there is no such obvious relationship in the cases of non- acute leukemia. The low- affinitive binding site of glucocorticoid (GCSBL) in the peripheral lymphocytes in 15 normal cases and 10 cases of acute leukemia, the Kd are 0. 8224±0. 241 μmol/l and 1. 067±0. 496 μmol/1 respectively, and the R0 ate 3614. 43±1233. 33 fmol/107 cell and 3488. 94±2272. 56 fmol/107 cell as well.