PREPARATIVE HPLC SEPARATION OF GLYCOCHOLIC ACID-(~(125)I-HISTAMINE)

A preparative HPLC separation method of glycocholic acid-(~(125)I-hist- amine) is described. The separation was carried out on YWG-CH reverse Phase column by using methanol: isopropanol: water (60: 5: 40, V/V) as mobile Phase. The separation was 30 min. The flow rate was 1ml/min, Using this method, glycocholic acid- (~(125)I-histamine)was Purified and satisfactorily used for RIA. The radiochemical Purity was 98％. The recovery efficiency was 99.1％.