Abstract: 103Pd is commonly used in brachytherapy, especially in the aspect of prostate cancer and choroidal melanoma treatment. At present, 103Pd is mainly produced by proton bombardment of rhodium target by cyclotron, and purified by extraction or ion exchange resin. In this work, the separation effects of AG 1-X 8 and AG MP-1 anionic resins were compared by using simulated samples under different chemical procedures, including such as hydrochloric acid concentrations of samples, elution methods, composition of desorption solutions. Under the optimized condition, using AG 1-X 8 resin, Rh matrix and other metal impurities are eluted by 6 mol/L HCl and 0.03 mol/L HCl. Pd is desorbed by mixing 1 mol/L ammonia and 1 mol/L ammonium chloride in equal volume, the recovery of Pd is nearly 100%, and the product has the largest Pd/Rh ratio. By this method, three batches of 103Pd were produced, and the radioactive concentration, specific activity and radionuclide purity of 103Pd were greater than 6 GBq/mL, 800 GBq/mg(EOS) and 99.9%, respectively.According to the requirements of the 2020 edition of the Chinese Pharmacopoeia for detection methods, the methodological validation of the analytical methods for the purity of radionuclide and the content of Pd elements was carried out in terms of exclusivity, limit of detection and limit of quantification, linearity, precision and accuracy, to ensure the accuracy and reliability of the test results.