Abstract: The radioisotope ^99mTc is widely used in the clinical diagnostic field of nuclear medicine, which is mainly obtained by β-decay of the parent nuclide ⁹⁹Mo in ⁹⁹Mo-^99mTc generator. Due to the shutdown of multiple reactors producing ⁹⁹Mo, the stable supply of fission ⁹⁹Mo is threatened. In addition, the production of fission ⁹⁹Mo may lead to nuclear proliferation. As a result, the raw materials for the fission-⁹⁹Mo-^99mTc generators decreased. Therefore, reactor irradiation and accelerator-based ⁹⁹Mo production technology have attracted the attention of various countries. New ⁹⁹Mo-^99mTc generators are also constantly being developed. This paper briefly discusses the current production status of low specific activity ⁹⁹Mo in China and the separation methods of low specific activity ⁹⁹Mo-^99mTc. Furthermore, the ⁹⁹Mo-^99mTc generator is also discussed, which is expected to provide some reference for the development of new ⁹⁹Mo-^99mTc generators in the future.