Abstract: The independent supply of isotopes is of great significance for the national economy and the construction of a Healthy China. To achieve the industrialization of domestically scarce isotopes.This study carried out the research and development of an isotope electromagnetic separator with four chambers. Through the analysis of the ion beam optics of the ion source, the magnetic field was calculated using the finite element method. Based on the physical calculation results, the design, manufacturing, and assembly of the equipment were completed, and the magnetic field measurement was carried out. As a result, the reception of stable isotopes such as ¹⁷⁶Yb and ⁸⁷Rb was successfully achieved, and the abundance results were satisfactory. This achievement marks the successful development of the isotope electromagnetic separator with four chambers, providing key support for the industrialization of domestically scarce isotopes. It is a great significance for promoting the independent supply of isotopes, boosting economic development, and contributing to the construction of a Healthy China. In the follow-up, the performance of the equipment can be further optimized to promote the development of the isotope industry.