Abstract: To meet the clinical application requirements, the Aβ-Amloid imaging agent ¹⁸F-AV45 was synthesized automatically using NEPTIS perform synthesis module .In the pre-written program, the AV105 was used as precursor to obtain the ¹⁸F-intermediate through nucleophilic substitution reaction with ¹⁸F⁻, followed by hydrolysis with hydrochloric acid, neutralization with base to obtain the crude product of ¹⁸F-AV45, which was next purified by solid-phase extraction and semi-preparative high-performance liquid chromatography (HPLC). Finally, ¹⁸F-AV45 injection solution was obtained through sterile filtration, followed by quality control analysis. The average production time of ¹⁸F-AV45 preparation was 60 min, the no-corrected efficiency was (32.1±2.1)% (n=5), the radiochemical purity was over 98%, and the appearance color, pH value, in vitro stability, bacterial endotoxin, and residual solvents all met the requirements. ¹⁸F-AV45 could be synthesized automatically and simply by NEPTIS perform module with good repeatability and the product meets the requirements for clinical use.