Abstract: Small-animal single-photon emission computed tomography-X-ray computed tomography (SPECT-CT) is an advanced imaging tool for preclinical research of single-photon radiopharmaceuticals. Its detection performance is the foundation of image quality and the reliability of experimental data. This study aims to evaluate the performance of Albira SI SPECT-CT in terms of advisable test range, linearity, stability, deviation of measurement results, related to the radioactive isotope ^99mTc, and to explore the authenticity and reliability of the scan results.The Albira SI SPECT-CT was subjected to quarterly quality control using the clinically commonly used single-photon radionuclide ^99mTc. The linearity, stability, and deviation of the measurement results were tested, and preliminary attempts were made at small-animal bone metabolism scanning. The device demonstrated a strong linear correlation between radioactivity and measurement results, exhibited high stability, and showed minimal variation when compared to commonly used radioactivity measurement devices.The Albira SI small animal SPECT-CT accurately reflects the distribution of the radioactive activity of the single photon isotope ^99mTc, showing excellent imaging of mouse bone metabolism, suitable for preclinical radiopharmaceutical research. The method for evaluating SPECT-CT performance established in this study is expected to provide a reference for the operation of similar equipment.