Abstract: The content of stannous chloride is one of the key indicators for the quality control of Technetium ^99mTc radiopharmaceutical injection Kits, which directly affects the clinical use of Technetium ^99mTc radiopharmaceuticals. Consequently, it is crucial to choose the appropriate analytical method for its accurate determination. By comparing the quality standards of radiopharmaceuticals included in the 2020 edition of the Chinese Pharmacopoeia and other related literature, the characteristics, determination principles and applications of commonly used methods for the determination of stannous chloride, including potentiometric analysis, spectrophotometry and high-performance liquid chromatography, were analyzed and summarized. This work provided technical reference for the establishment of a more scientific and rational quality standard for Technetium ^99mTc radiopharmaceutical injection kits.