Abstract: ⁹⁹Mo is an important nuclide widely used in nuclear medicine However, due to the safety risks in the conventional process of producing ⁹⁹Mo by fission of highly enriched uranium, reliable ⁹⁹Mo production methods were developed. Column chromatography has great advantages in separation of ⁹⁹Mo and ^99mTc, and the key is the material of the column, whose adsorption capacity for ⁹⁹Mo is related to the preparation of a new generation of molybdenum technetium generator in the future. In this paper, the adsorption and separation of medical isotope ⁹⁹Mo were reviewed, the production method of ⁹⁹Mo, the separation method of ⁹⁹Mo and ^99mTc were introduced, and the adsorption materials with certain adsorption effect on Mo were introduced, which provided a reference for the future preparation of ⁹⁹Mo-^99mTc generator by adsorption of ⁹⁹Mo with low specific activity.