Abstract: Usually, radon detector must be calibrated every year, commonly used solid radon source calibration method and radon chamber calibration method. The former has the problems of radon source device aging, complex operation and radioactive substances vulnerable to transport control. The latter has problems such as calibration time principal and inability to achieve on-site calibration. Based on the above facts, this paper proposes an experimental method for the calibration of radon measuring instrument using uranium waste rock, which is used to realize the field calibration of seismic radon measuring instrument. The results show that the radon concentration control method has high accuracy and stability, and the relative error between the output gas radon concentration and the preset target is 1%. The comparison between the calibration experiment of uranium mine waste rock and the calibration experiment of standard radon chamber shows that the calibration values of the calibration coefficient of the two are in good agreement, the relative error is only 1.14%, and the normalized error E_n is only 0.135 6, which is within the expected range. It can provide theoretical support for the subsequent development of portable radon detector calibration device.