Abstract: As one of the important ways to prepare radioisotopes, the role and status of accelerator produced nuclides have become increasingly prominent after entering the new century. In the present paper, a brief overview on development status of radioisotope preparation technology by accelerators at home and abroad was performed. In particular, the research progress of ¹⁸F, ⁶⁴Cu, ⁶⁸Ge/⁶⁸Ga, ⁸⁹Zr, ¹¹¹In, ²¹¹At, ²²⁵Ac and other important medical radioisotope preparation technologies are introduced. The application prospects of isotopes prepared by domestic accelerators are also prospected.