Abstract: As the decay daughter of ⁹⁹Mo, ^99mTc is the most widely used radioisotope in the field of nuclear medicine. Recently, the major ⁹⁹Mo production reactors in the world are facing problems such as unplanned maintenance, shutdown and decommission, resulting in the unstable supply of ⁹⁹Mo. Therefore, many developed countries have been actively exploiting alternative technologies for ⁹⁹Mo production. In this paper, various ⁹⁹Mo-production technologies by particle accelerator instead of reactor were introduced, and the research progress of producing ⁹⁹Mo based on electron accelerator was summarized at home and abroad. The accelerator performance index and the key technologies in the production process were discussed in detail, such as the design of irradiation device and target, separation of ^99mTc, recovery of ¹⁰⁰Mo and waste management. In addition, some suggestions were put forward for the basic and applied research of relevant technologies in China and the commercialization of ⁹⁹Mo production by electron accelerator.