Abstract: In this paper the single-use protective clothing for medical use was sterilized by γ-ray irradiation. Compared with the non-irradiated control, the apparent morphology of PP melt-blown fiber and amboss had no fracture. According to the national standard of GB/T 19082-2009, the mechanical properties, resistance to synthetic blood penetration, resistance to water penetration and filtering efficiency were measured. The results showed that the post-irradiation effects did not decrease the resistance to synthetic blood penetration or water penetration. Moreover, the filtering efficiency remained higher than 99.8%. On the other hand, the mechanical properties were reduced significantly due to the degradation of poly chain of PP induced by γ-ray irradiation. It has been assured that the important properties of single-use protective clothing for medical use are still above the quality level after γ-ray irradiation sterilization process. which can supply reference for the produce single-use protection of clothing of medical purpose at high quality.