Abstract: The electron beam irradiation technology was used to decompose oxytetracycline residue, and the oxytetracycline residue and its physical and chemical properties before and after irradiation were analyzed by LC-MS, GC-MS, ICP-MS, fourier infrared spectroscopy and other analytical techniques. The results showed that electron beam irradiation technology could effectively degrade the residual oxytetracycline, and the removal rate of oxytetracycline reached 76.33% at 200 kGy. The main functional groups in the bacterial residue were CC and O—H, as the absorbed dose increased, the absorption peak intensity first increased and then decreased. After irradiation, the pH value of the bacterial residue had a decreasing trend, the concentration of halide ions increased, the cyanide was completely degraded, and the concentration of metal ions changed little, the result mainly due to the reaction of organic matter with active particles such as ·OH, e^-_aq, ·H produced by electron beam irradiation, various factors were lower than the limit requirements of the hazardous waste identification standards. The research results could provide a theoretical basis for the treatment and resource utilization of antibiotic residues.