Abstract: The design, planning and implementation of the first time uranium isotope detection project in inter-laboratory comparisons of nuclear fuel industry were introduced, the primary certified reference materials uranium isotopic of U₃O₈ was selected as split sample pair, Z-score and the allowable error limit (E) were used as the capability statistics to calculate and analyze the results of the inter-laboratory comparisons of uranium isotope enrichment, analysis and suggestions were made on the problem or dissatisfaction of the measurement results. By carrying out the first time comparison of uranium isotope project, the project of nuclear industry laboratories capability verification was expanded and the testing level of the laboratories were improved further. The technical foundation and quality assurance for the importance of uranium isotope measurement in production of nuclear fuel cycle was provided.