Abstract: The use of the chromatographic method to extract ⁹⁹Tc^m from the low specific activity ⁹⁹Mo solution is an effective way to obtain medical isotope ⁹⁹Tc^m that meeting clinical requirements. It has been reported that the separation technologies must use strong acid or strong alkaline solutions, which require additional treatments. Exploring the method of separating technetium from neutral molybdenum solution can simplify the treatment process. A kind of activated carbon fiber was used to extract technetium from neutral low specific activity ⁹⁹Mo (＜0.2 Ci/g Mo) in 1 mol/L NaCl solution. Neutral eluent was used to desorb technetium throughout the process, and the separation of molybdenum and technetium was realized. The recoveries of ⁹⁹Tc^m were more than 90%. The obtained sodium pertechnetate ⁹⁹Tc^m injection met the requirements of the Chinese Pharmacopoeia without pH adjustation, and the labeling rate of MIBI and MDP for met the Chinese Pharmacopoeia claim. The results show that the extraction of ⁹⁹Tc^m from ⁹⁹Mo with low specific activity (<0.2 Ci/g Mo), independent intellectual property rights and obvious advantages is formed, which provides technical reserves for potential methods of preparing ⁹⁹Tc^m.