Abstract: Radioisotopes play an important role in the development of national economy and health care. In the present paper, a brief overview on development of radioisotopes preparation technology in the past three decades in China was performed. The development of preparation technology for some important radioactive isotopes, such as ¹⁴C, ⁶⁰Co, ⁹⁹Mo, ^123/125/131I, ¹⁷⁷Lu was described. Based on the analysis of the existing problems on the development of radioisotopes preparation technology, solutions to this issue are pointed out, which will be expected to boost the development of radioisotope preparation technology in China and finally serve the domestic radioisotope production in future.