Abstract: For better understanding the traits and advantages of the isotopes tracing technology, the research hotspot fields of isotopes technology in agricultural sciences were reviewed. ¹⁵N and ³²P tracing technology being applied to study the nutrient transformation, provided important theory support for efficient utilization of nutrients. ¹³C and ¹⁵N tracing technology used extensively in soil organic carbon cycling and greenhouse emissions, showed prominent advantages among traditional technologies. ¹⁴C tracing technology performed important technical support for environmental impact assessment of new agricultural chemicals. Isotopic fingerprint technology, such as stable isotope ratio and δ¹³C, provided an important tool for analysis of environmental behavior of agricultural pollutants and its traceability. This technology also provided technical ideas for tracing the source of high quality agricultural produce. ¹⁰⁹Cd and ⁶⁵Zn tracing technology provided indispensible technical support for studying environmental behavior of Cd and Zn. ⁷Be and ¹³⁷Cs tracing technology also used as a unique tool for studying ecosystem stability.