Abstract: There are many kinds of methods for preparing graphite target for ¹⁴C dating by accelerator mass spectrometer (AMS). Increasing the yield of graphite is of great significance for optimizing the preparation processes of graphite target and improving the performance of graphite target. In this paper, the studies relating to the graphite target preparation by Zn-TiH₂ method were cited as theoretical foundations. The relationship between the yield of graphite and isotopic fractionation was proved by the relevant researches. Different calculation methods of graphite yield were compared, and suggestions for improvement were put forward. The factors influencing the yield of graphite in experimental processes were discussed. The optimum experimental conditions for preparing graphite target by Zn-TiH₂ method were briefly summarized.