Abstract: The demand for stable isotopes has increased in the field of medical diagnosis, biopharming, quantum communication and so on. Natural carbon has two stable isotopes: ¹²C (98.89%) and ¹³C (1.11%). ¹³C in high abundance is mainly used in tracer field. For example, ¹³C can be manufactured in the form of urea for Helicobacter Pylori diagnosis without any radioactivity or toxicity. As much more focus has been paid on safety and health, ¹³C reveals big advantage over other radioactive tracer isotopes. The radically increased demand for ¹³C put forward higher requirements on both the capacity and maximal abundance on ¹³C production. Rectification is the only method which has been used for large-scale industrial production of ¹³C at present all over the world, while China has not yet mastered the technology to produce ¹³C in high abundance under industrial scale. Considering gas centrifugation method owns the advantages of low energy consumption and large single-stage separation factor, studies on centrifugal separation of carbon isotopes were carried out. Using heptafluoropropane (C₃HF₇) as processing gas, single-centrifuge experiments were done based on domestic gas centrifuges. Separation products were analyzed by a MAT-253 gas mass spectrometer. As a result, the separation factor and single-centrifuge separative power under different working conditions were obtained. The single-centrifuge separation factor could reach 1.12. Based on the results of single-centrifuge separation experiments, the working condition with the maximum separative power was selected as the calculation parameters, then cascade calculation of the enrichment of ¹³C isotope was conducted by the matched abundance ratio cascade (MARC). The design parameters of centrifugal cascades for enrichment of ¹³C were worked out. Through three-time separations by MARC, with stage number of 30, 60 and 75, respectively, ¹³C isotope could be enriched from natural abundance to above 30% in abundance. With overall consideration of single centrifuge experiments and cascade calculation, the feasibility of separating carbon isotopes by gas centrifuge using heptafluoropropane as processing gas has been demonstrated. The study lays a foundation for carbon isotopes production by gas centrifugation method.