Abstract: The use of ¹⁴C as a tracer for oil field tracer has important value. Applying accelerator mass spectrometry to measure ¹⁴C-labeled samples obtained from the oil field tracer was presented. In order to meet the requirements of large sample size and rapid analysis, a simple sample preparation process for CaCO₃ had been established. The tracer samples used in the experiments came from two production wells. For each sample, it was divided evenly into two parts, one for carbonate extraction and another for urea carrier extraction. The AMS measurement results showed that the content of tracer ¹⁴C was slightly higher than the background value in the 1 # carbonate series, and the trend was not obvious. There might be tracer ¹⁴C in the 2 # carbonate series，but it did not match the ³H tracer results.The analysis indicated that the carbonate series might be contaminated during sample preparation. Compared with ³H, the AMS measurement results of ¹⁴C indicate that the tracer dose requested can be significantly reduce.