Abstract: As an important complementary imaging agent for ¹⁸F-FDG, ¹⁸F-fluoroethyl-choline (¹⁸F-FECH) has been demonstrated to be promising in brain and prostate cancer imaging. By using domestic PET-FDG-TI-I CPCU synthesizer, ¹⁸F-FECH was synthesized by different reagents and consumable supplies. The C18 column was added before the product collection bottle to remove K_2.2.2. The ¹⁸F-FECH was synthesized by PET-FDG-IT-I synthesizer efficiently about 30 minutes by radiochemical yield of 42.0% (no decay corrected, n=5), and the radiochemical purity was still more than 99.0% after 6 hours. The results showed the domestic PET-FDG-IT-I synthesizer could semi-automatically synthesize injectable ¹⁸F-FECH in high efficiency and radiochemical purity.