Abstract: Biodistributions of micro-PET tracer ¹⁸F-FDG, ¹⁸F-RGD, and ¹⁸F-FET in the LN229 induced glioma nude mice were performed using micro-PET technology. Tyrosine and NOTA modified c(RGDyK)₂ were employed as precursors for the synthesis of ¹⁸F-FET and ¹⁸F-RGD. Glioma model was established via intracerebral injection of LN229 cells and the biodistribution in tumor and major organs at 30 min and 60 min postinjection of ¹⁸F-FDG, ¹⁸F-RGD, and ¹⁸F-FET were determined by Gamma counting, respectively. The results showed more than 98% purity for ¹⁸F-FET and ¹⁸F-RGD. After 60 min injection of three radiotracers, the uptake values of ¹⁸F-FDG, ¹⁸F-RGD, and ¹⁸F-FET in tumor and brain were 4.89±0.65%ID/g (（2.72±0.76）%ID/g), 2.10±0.32%ID/g (（0.23±0.01）%ID/g), and 3.02±0.64%ID/g （(0.74±0.12）%ID/g), respectively. In addition, the ratio of tumor to brain of ¹⁸F-FDG, ¹⁸F-RGD, and ¹⁸F-FET were determined as 0.64±0.07（1.80±0.32）、 8.22±1.03（9.13±1.21） and 2.15±0.48（4.08±0.92）. Micro-PET imaging also represented a clearly visualized tumor circumscription. In conclusion, micro-PET combined with ¹⁸F-FET and ¹⁸F-RGD could be a promising technique for the diagnosis and research of glioma.