<sup>125</sup>I-rAncrod在大鼠体内的组织分布与代谢

于秋菊; 朱晓霞; 王德才; 孟志云; 甘慧; 顾若兰; 吴卓娜; 郑颖; 李俭; 窦桂芳

doi:10.7538/tws.2015.28.02.0107

¹²⁵I-rAncrod在大鼠体内的组织分布与代谢

1.
泰山医学院,山东泰安271000
2.
军事医学科学院野战输血研究所,北京100850

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- 刊出日期: 2015-05-19

Tissue Distribution and Metabolism of ¹²⁵I-rAncrod in Wistar Rats

1.
Taishan Medical University, Taian 271000, China
2.
Institute of Transfusion Medicine, Academy of Military Medical Science, Beijing 100850, China

摘要

摘要: 为研究¹²⁵I-rAncrod（1.59 μg•kg^-1）在Wistar大鼠器官和组织分布以及粪尿和胆汁的代谢情况，采用放射性¹²⁵I标记重组安克洛酶（¹²⁵I-rAncrod）结合分子排阻色谱法（size exclusion-chromatography,SEC）及γ-计数法测定不同时间组织及体液样品中¹²⁵I-rAncrod的含量。结果显示，放射性药物浓度主要累积在甲状腺和胃肠道内，肝肾次之，而脑、脊髓、脂肪、肌肉中药物浓度一直处于较低的水平。Wistar大鼠尾静脉注射¹²⁵I-rAncrod后，SEC色谱图显示到48 h粪尿和胆汁中均检测不到原形药物。结果表明，大鼠尾静脉注射¹²⁵I-rAncrod后在全身各器官和组织分布广泛，代谢较完全。
- ¹²⁵I标记重组安克洛酶 /
- 组织分布 /
- 代谢
Abstract: To study the tissue distribution and metabolism of radioactivity in rats after single intravenous administration of ¹²⁵I-rAncrod（1.59 μg•kg^-1）. The concentration of ¹²⁵I-rAncrod in various tissue and body fluids were determined by ¹²⁵I labeled rAncrod combined with size exclusion chromatography （SEC） and γ-counting methods. The concentration of ¹²⁵I-rAncrod predominantly accumulated in the thyroid and gastrointestinal and also be found in the kidney and liver in fair amounts, but in brain, spinal cord, muscle and fat was at a very low level. After single intravenous administration (chemical dose 1.59 μg•kg^-1) of ¹²⁵I-rAncrod to Wistar rats, the samples of feces, urine and bile were analyzed by SEC. The result was that parent drug was not existed in feces, urine and bile in 48 h. The results showed that ¹²⁵I-rAncrod was distributed widely in rats, metabolic process was complete.
- ¹²⁵I-rAncrod /
- tissue distribution /
- metabolism

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参考文献(14)

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125I-rAncrod在大鼠体内的组织分布与代谢

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Tissue Distribution and Metabolism of 125I-rAncrod in Wistar Rats

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目录

¹²⁵I-rAncrod在大鼠体内的组织分布与代谢

Tissue Distribution and Metabolism of ¹²⁵I-rAncrod in Wistar Rats