D<sub>12</sub>-孔雀石绿的合成

D₁₂-孔雀石绿的合成

摘要: 以CD₃OD为起始原料，经甲基化、缩合、氧化等步骤合成稳定同位素标记D₁₂-孔雀石绿。以消耗CD₃OD计算，稳定同位素标记D₁₂-孔雀石绿的总收率为50.1%。产品结构经高效液相色谱（HPLC）、质谱（MS）及核磁共振波谱（NMR）等仪器表征确定，D₁₂-孔雀石绿色谱纯度高于99.0%，氘同位素丰度大于99.0%，结果表明，D₁₂-孔雀石绿可作为食品安全领域检测用同位素内标试剂。

Abstract: The efficient synthesis of stable isotope labeled D₁₂-Malachite Green with CD₃OD as the precursor by methylation, condensation and oxidation was presented. The yields of D₁₂-Malachite Green was 50.1 % based on CD3OD consumed. The product was ideatified by HPLC, MS, and NMR to be the target compound. Its chemical purity and isotopic enrichment were higher than 99.0 atom%D. The result showed that the D₁₂-Malachite Green could be used as internal standard in the field of food safety.