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99Tcm-膜连蛋白V在下腔静脉血栓模型家兔体内生物分布

Study of 99Tcm-Annexin V Distribution in Inferior Vena Cava Thrombus Models of Rabbits

    摘要
  • 摘要: 观察99Tcm-膜连蛋白V（Annexin V）在下腔静脉血栓模型家兔体内分布及新鲜与陈旧静脉血栓对99Tcm-Annexin V的摄取。将15只家兔随机分为新鲜血栓组、陈旧血栓组及对照组，每组5只。新鲜血栓组与对照组于术后1 d、陈旧血栓组术后14 d注射4 mCi 99Tcm-Annexin V ，注射1 h后处死，两血栓组取血栓、对照组取对应下腔静脉血管，另均取血液、头侧下腔静脉、后肢肌肉、胃、心、肺、肝、肾、脾、骨及小肠组织，称重、测量放射性计数，计算放射性摄取率（％ID/g），两血栓组计算血栓与血液、血栓部位下腔静脉血管、头侧下腔静脉血管、肌肉的放射性摄取比。血栓标本行HE染色。采用SPSS17.0软件对新鲜血栓组与陈旧血栓组结果进行t检验。结果显示，新鲜血栓组血栓放射性摄取率为（0.018 94±0.002 16）%ID/g，高于陈旧血栓组（0.004 73±0.001 28）%ID/g，差异具有统计学意义(t=12.66，P<0.05)。新鲜血栓组血栓与血液、血栓与血栓部位下腔静脉血管、血栓与头侧下腔静脉血管、血栓与肌肉的放射性摄取比（T/NT）分别为3.42±1.06、26.32±13.60、31.23±16.00、111.62±52.23，均高于陈旧血栓组（0.98±0.09、5.12±2.01、6.25±2.38、21.82±5.93），差异有统计学意义（t分别为5.14、3.44、3.45、3.82，P均<0.05）。新鲜血栓组血栓病理示为新鲜混合血栓，陈旧血栓组血栓为机化混合血栓。以上结果表明：99Tcm-Annexin V有望应用于急性新鲜静脉血栓显像，并用于新鲜与陈旧静脉血栓的鉴别。

     

    Abstract: To study 99Tcm-Annexin V distribution in inferior vena cava thrombus models of rabbits and uptake of 99Tcm-Annexin V in fresh and old venous thrombus. Rabbits(n=15) were randomly grouped into 3 groups(the fresh thrombus group，old thrombus group，and control group). The rabbits of two thrombus groups developed inferior vena cava thrombus models by operations. The control group received sham operation. The fresh thrombus group and control group rabbits were injected 99Tcm-Annexin V after operating 1d; the old thrombus group 14 d. After 1 h all rabbits were killed by injecting overdose pentobarbital sodium. The thrombus (or the inferior vena cava about 3cm below inferior pole of right kidney level in the control group rabbits)，blood，thrombus area inferior vena cava，head lateral inferior vena cava (except the control group)，thigh muscle，stomach，myocardium，pulmonary，liver，kidney，spleen，bone and small intestine were obtained from all group rabbits. The ex tissue and blood were weighed and measured by a Well-type detector. The percentage of the injected dose per gram of ex tissue (or blood) was calculated by the above data. The thrombus to blood，thrombus area inferior vena cava，head lateral inferior vena cava and thigh muscle ratios were calculated by percentage of the injected dose per gram of ex tissue (or blood). The test was used to compare the fresh thrombus group and old thrombus group by SPSS 17.0. The percentage of the injected dose per gram of thrombi（0.018 94±0.002 16%ID/g）in the fresh thrombus group was higher than the old thrombus group (0.004 73±0.001 28%ID/g), P<0.05. The thrombus to blood，thrombus area inferior vena cava，head lateral inferior vena cava and muscle ratios (3.42±1.06，26.32±13.60，31.23±16.00，111.62±52.23) in the fresh thrombus group were higher than the old thrombus group(0.98±0.09，5.12±2.01，6.25±2.38，21.82±5.93), P<0.05 for all. All the thrombi of the fresh thrombus group were confirmed fresh mixed thrombi by Hematoxylin-eosin stains. All the thrombi of the old thrombus group were confirmed old mixed organized thrombi by Hematoxylin-eosin stains. 99Tcm-Annexin V may be used to venous thrombus imaging and discriminate fresh venous thrombus from old one by imaging.

     

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