Abstract: With the wide use of positron emission tomography (PET) imaging for the detection of cancer, neurological diseases, and cardiovascular diseases, peptides labeled with positron-emitting radionuclide (¹⁸F) as the promising PET tracers have been made rapid development in recent years. There are many methods for ¹⁸F-labeled peptides. According to the characteristics of the labeling reactions, this review summarized the methods of ¹⁸F-labeled peptide as follows： 1） ¹⁸F-labeled prosthetic group method; 2） solid-phase labeling method; 3） ¹⁸F-AlF labeling method; 4） click reaction; 5） ¹⁸F-¹⁹F isotope exchange method; 6） aromatic electrophilic or nucleophilic labeling method; 7） the other labeling methods. Furthermore, these methods’ advantage and disadvantage were analyzed.